Structural studies on human glutathione S-transferase pi. Substitution mutations to determine amino acids necessary for binding glutathione.
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Inhibition and recognition studies on the glutathione-binding site of equine liver glutathione S-transferase.
Equine liver glutathione S-transferase has been shown to consist of two identical subunits of apparent Mr 25,500 and a pl of 8.9. Kinetic data at pH 6.5 with 1-chloro-2,4-dinitrobenzene as a substrate suggests a random rapid-equilibrium mechanism, which is supported by inhibition studies using glutathione analogues. S-(p-Bromobenzyl)glutathione and the corresponding N alpha-, CGlu- and CGly-sub...
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Glutathione S-transferases (GSTs) are dimeric proteins that play a major role in cellular detoxification. The GSTs in mosquito Anopheles dirus species B, an important malaria vector in South East Asia, are of interest because they can play an important role in insecticide resistance. In the present study, we characterized the Anopheles dirus (Ad)GST D3-3 which is an alternatively spliced produc...
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Class-Pi of glutathione s-transferases (GST-Pi) is the specific form of GSTs that are known to participate particularly in the mechanisms of resistance to drugs and carcinogens. This class of the enzyme is referred to as class-P or class-Pi or class π. The accepted terminology in this review article is class-Pi. In this article following a brief description of identified molecular forms of GSTs...
متن کاملReversible Conjugation of Ethacrynic Acid with Glutathione and Human Glutathione S-Transferase PI-1
The reversibility of the conjugation reaction of the diuretic drug ethacrynic acid (EA), an a,~-unsaturated ketone, with glutathione and glutathione S-transferase PI-1 (GST PI-I) has been studied. When the glutathione conjugate of EA was incubated with a 5-fold molar excess of Nacetyi-L-cysteine or GST PI-I, a time-dependent transfer of EA to N-acetyI-L-cysteine or GST PI-1 was observed. With i...
متن کاملReversible Conjugation of Ethacrynic Acid with Glutathione and Human Glutathione S-Transferase PI-1
The reversibility of the conjugation reaction of the diuretic drug ethacrynic acid (EA), an a,~-unsaturated ketone, with glutathione and glutathione S-transferase PI-1 (GST PI-I) has been studied. When the glutathione conjugate of EA was incubated with a 5-fold molar excess of Nacetyi-L-cysteine or GST PI-I, a time-dependent transfer of EA to N-acetyI-L-cysteine or GST PI-1 was observed. With i...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)37051-6